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Determination of forsythin content in Niuhuang Huadu tablets by HPLC |
Hits 1807 Download times 1729 Received:November 20, 2010 |
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DOI
10.11656/j.issn.1673-9043.2011.02.16 |
Key Words
HPLC;Niuhuang Huadu tablets;forsythin;content |
Author Name | Affiliation | MENG Fan-ying | Tianjin University of TCM, Tianjin 300193, China | ZHANG Li-fang | Tianjin Tongrentang Co Ltd Tianjin 300132, China | HOU Ting | Xiehe Stem Cell Jine Co Ltd., Tianjin 300384, China | 王佳 | Tianjin Tongrentang Co Ltd Tianjin 300132, China | 卢珑 | Tianjin University of TCM, Tianjin 300193, China |
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Abstract
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[Objective] To establish HPLC method for determination of forsythin content in Niuhuang Huadu tablets. [Methods] The determination was performed on C18 column,the mobile phase consisted of acetonitrile-water (23:77) with flow rate at 1.0 mL/min and detection wavelength at 230 nm under room temperature. [Results] Forsythin had good linear relation in the range of 0.194 6~2.919 0 μg (r=0.999 9),the average recovery was 99.90% (RSD=1.69%). [Conclusion] The method can be used to control the quality of Niuhuang Huadu tablets. |
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