|
| The effect of Dioscorea nipponica Makino on bone marrow T-lymphocyte subsets in mice with aplastic anemia |
| Hits 1410 Download times 1822 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2011.03.13 |
| Key Words
Dioscorea nipponica Makino;aplastic anemia;T-cell;subgroup |
| Author Name | Affiliation | | ZHANG Wei-feng | Tianjin University of TCM, Tianjin 300193, China
| | LIU Bao-shan | The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China |
|
| Abstract
|
| [Objective] To observe the effect of Dioscorea nipponica Makino in treating in aplastic anemia and the expression of CD3+,CD4+,CD8+on bone marrow mononuclear cells.[Methods] The 105 healthy ICR mice were chosen.The 15 mice were served for normal control.The other 90 mice were served for inducing aplastic anemia (AA) with Sun Jiyuan methods.After model identification,successful model mice were randomly divided into 6 groups (model group;small dosage group;middle dosage group;large dosage group;Cyclosporine A group;Tripterygium Glycoside group,n=15 each).From the 1st day to the 14+th day,these mice were gavaged with different methods daily.Blood routine examination was performed on the 15th day.Bone marrow mononuclear cells for cell culture were extracted,and CD3+,CD4+,CD8+cells were detected with Flow Cytometer after 24+ hours.[Results] Compared with the model group,the expression of CD3+,CD4+,CD4+/CD8+in treatment groups were significantly increased (P<0.05). CD8+obviously reduced,and it didn't have significant difference in normal group,middle dosage group and large dosage group (P>0.05),but it had significant difference in middle dosage group and large dosage group and the two control groups.[Conclusion] Dioscorea nipponica Makino can regulate the balance of T-lymphocyte subsets in mice with AA. |
|
|
|
|
|
