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| Determination of 6-gingerol in ginger by high performance liquid chromatography |
| Hits 2204 Download times 2824 Received:August 29, 2011 |
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| DOI
10.11656/j.issn.1673-9043.2012.01.13 |
| Key Words
ginger;6-gingerol;HPLC |
| Author Name | Affiliation | | LI Li | Tianjin University of TCM, Tianjin 300193, China | | ZHU Xiao-wei | Tianjin University of TCM, Tianjin 300193, China | | LI Shuang | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To establish a HPLC method for the determination of 6-gingerol in ginger.[Methods] Ginger was extracted by heat reflux with 80% methanol for 2 hours,the drug ratio was 1:100,and the Hypersil C18 ODS (250 mm×4.6 mm,5μm) column was used to determine.The mobile phase consisted of methanol-water-isopropyl-alcohol-glacial acetic acid (38:36:12:4);The flow rate was 0.8 mL/min.The detection wavelength was 280 nm.The column temperature was kept at 34℃.[Results] The linear range was between 0.021 6~0.432 0μg.And the average recovery of the method was (100.79±2.84)% and the RSD was 2.84%.[Conclusion] The results show that this assay method is simple and accurate.And the method can be used to determine the contents of 6-JF in Zingiber of-ficinale Rose. |
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