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| Effects of compound Kushen injection on expression of Bcl-2/Bax and P27 in human endometrial cancer HEC-1B cell |
| Hits 2456 Download times 2219 Received:February 18, 2012 |
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| DOI
10.11656/j.issn.1673-9043.2012.02.10 |
| Key Words
compound Kushen injection;human endometrial cancer;anti-tumor;apoptosis;experimental study |
| Author Name | Affiliation | | ZHANG Wen-jin | National Engineering Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100075, China
Tianjin University of TCM, Tianjin 300193, China | | PENG Yao | National Engineering Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100075, China
Beijing University of TCM, Beijing 100102, China | | LIAN Zeng-lin | National Engineering Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100075, China | | JIN Ran | National Engineering Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100075, China | | LI Jin-sheng | National Engineering Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100075, China | | HAI Li-na | Beijing Zhendong Guangming Pharmaceutical Institute Co.Ltd, Beijing 100120, China |
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| Abstract
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| [Objective] To explore the effects of compound Kushen injection (Yanshu) on Bcl-2/Bax,P27 expression in endometrial cancer HEC-1B cell.[Methods] Using MTT method the inhibition of HEC-1B cell proliferation treated by compound Kushen injection, Kushen injection, Baituling injection and DDP injection was detected. Using RT-PCR method their expression of Bcl-2/Bax and P27 was also detected.[Results] Compound Kushen injection and Kushen injection could inhibit the proliferation of HEC-1B cell and the effect was dosedependent. The expression of Bcl-2 gene was reduced significantly while Bax and P27 gene were risen on mRNA level treated for 48 h by compound Kushen injection, Kushen injection and DDPC injection (P<0.05).[Conclusion] Compound Kushen injection and Kushen injection can inhibit the expression of Bcl-2 and promote the expression of Bax and P27. Compound kushen injection and it's active ingredients Kushen can reduce Bcl-2 expression in HEC1B cell while rise Bax and P27 gene on mRNA level. |
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