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| Effects of Yupao Yin on expression of CX43 in ovarian granulosa cells of rats and secretion function |
| Hits 1445 Download times 2063 Received:May 12, 2012 |
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| DOI
10.11656/j.issn.1673-9043.2012.03.06 |
| Key Words
Yupao Yin;ovarian granulosa cells;connexin43;E2;experimental study |
| Author Name | Affiliation | | JIN Zhe | Dong Fang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China | | LI Nan | Beijing University of Chinese Medicine, Beijing 100029, China | | LIU Yan-xia | Dong Fang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China |
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| Abstract
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| [Objective] To assess the effects of Yupao Yin on expression of CX43 in ovarian granulosa cells(GC) of rats and secretion quantity of E2.[Methods] Granulosa cells are isolated from rats ovaries and cultured for 48 hours,followed by culture in medium system containing FSH(50 ng/mL)and different dosage of Yupao Yin for 48 hours.The expression of CX43 is measured by western blot and the secretion quantity of E2 is performed by radioimmunoassay.[Result] Yupao Yin can promote secretion of E2 and the secretion quantity of E2 gradually increases with the increase of the dosage.Yu pao Yin can significantly stimulate the expression of CX43.Amomg them the effect of high dosage group is similar with FSH group.[Conclusion] Yupao Yin obviously promote the expression of CX43 and secretion of E2 of ovarian GC of rats.It is showed that Yupao Yin can promote proliferation of GC and enhance the ability of GC anti-apoptosis through up-regulation the expression CX43.And then to promote the development of follicular. |
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