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| 5-Azacytidine combined with salvianolic acid B can promote differentiation from bone marrow Mesenchymal stem cells derived from rats to cardiomyocytes |
| Hits 2765 Download times 1903 Received:November 16, 2012 |
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| DOI
10.11656/j.issn.1673-9043.2013.01.07 |
| Key Words
5-Azacytidine;Salvianolic acid B;MSCs;differentiation |
| Author Name | Affiliation | E-mail | | GAO Qing | Tianjin University of TCM, Tianjin 300193, China | | | JI Hong | Tianjin University of TCM, Tianjin 300193, China | | | HU Xian-tong | Tianjin University of TCM, Tianjin 300193, China | | | WANG Yi-jing | Tianjin University of TCM, Tianjin 300193, China | | | FAN Ying-chang | Tianjin University of TCM, Tianjin 300193, China | fych@tjutcm.edu.cn |
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| Abstract
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| [Objective] To discuss the role of 5-Azacytidine and salvianolic acid B in inducing differentiation of Mesenchymal stem cells (MSC) derived from rats into cardiomyocytes. [Methods] A method was established by culturing MSCs from the bone marrow of rat. The well-conditioned MSCs were divided into four groups: the control group, the 5-Azacytidine (5-aza) group, the salvianolic acid B (salB) group, the 5-Azacytidine combined with salvianolic acid B (5-aza+salB) group. Two weeks after inducing the mRNA and protein expression of GATA-4 and α-MHC were detected by QPCR and Western Blot in each group. [Results] The mRNA and protein expression of GATA-4 and α-MHC in each induced group were higher than control group, and the 5-aza+salB group was significantly higher than 5-aza and salB group. [Conclusion] 5? -Azacytidine combined with Salvianolic acid B can promote differentiation rate of cardiomyocytes from Mesenchymal stem cells of bone marrow derived from rats. |
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