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| Determination of Mangiferin content in mango tree leaves |
| Hits 2244 Download times 2494 Received:November 15, 2012 |
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| DOI
10.11656/j.issn.1673-9043.2013.01.12 |
| Key Words
mango tree leave;Mangiferin;HPLC;determination |
| Author Name | Affiliation | E-mail | | GUO Ling-ling | Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China | | | ZHANG Yi | Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China | | | LIU Er-wei | Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China | | | HAN Li-feng | Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China | | | WANG Tao | Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin, Tianjin 300193, China | wangt@263.net |
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| Abstract
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| [Objective] To establish the determination method of Mangiferin content in mango tree leaves for analyzing samples collected in different season. [Methods] HPLC method was performed on Cosmosil ODS column. Mobile phase: CH3CN-1% HAc (15:85), flow rate: 1 mL/min, column temperature: 25 ℃, detection wavelength: 254 nm. The samples of mango tree leaves were collected in different seasonin from Hainan province for determination of mangiferin. [Results] The method showed a linear relationship between peak area and injection amount at the range of 0.069 5～2.224 0 μg for mangiferin. The average recovery rate was 96.77%, RSD=1.19%. [Conclusion] This HPLC method for the content determination of mangiferin shows good sensitivity, accuracy and repeatability. |
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