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| Experimental research of extract chitin from Meyen and analyse antibacterial of chitosan |
| Hits 2954 Download times 2104 Received:February 15, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2013.02.09 |
| Key Words
Locusta migratoria manilensis(Meyen);chitin;chitosan;antibacterial;TEM |
| Author Name | Affiliation | | LUO Ping | Tianjin Medical College, Tianjin 300222, China
Nankai University, Tianjin 300071, China | | SUN Xiao-yan | Nankai University, Tianjin 300071, China | | JING Ying-jun | Nankai University, Tianjin 300071, China | | SHAN Ying | Nankai University, Tianjin 300071, China | | LI De-sen | Nankai University, Tianjin 300071, China | | DU Rong-qian | Nankai University, Tianjin 300071, China | | LÜ Xian-yu | Nankai University, Tianjin 300071, China |
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| Abstract
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| [Objective] To choose the best condition of extract chitin from Meyen, and discuss antibacterial activities and mechanism of different degree of deacetylation and different molecular weight chitosan were discussed. [Methods] The various content N of chitin were obtained by change experiment condition Antibacterial experiment adopted method of antibacterial ring and bacterial shape were observed by TEM.[Results] Nine chitin containing the various N were obtained. Chitosan with molecular weight between 15~95 kD and 90%～95% deacetylation expressed high antibacterial activity.[Conclusion] The best condition of extract chitin from Meyen is as follows: NAOH' consistency 8%, 100℃, 6 h, chitosan has antibacterial activities to 5 bacteria lower molecular weight and deacetylation chitosan lower antibacterial activities. Antibacterial mechanism of chitosan is deduced because of destroying cytoderm. |
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