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| Impact of D-galactose’s dose and gender of mice on aging model |
| Hits 2579 Download times 2276 Received:April 28, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2013.03.06 |
| Key Words
D-galactose;aging;learning and memory ability;gender;growth factor |
| Author Name | Affiliation | E-mail | | LIN Ying-xue | Tianjin University of TCM, Tianjin 300193, China | | | ZHUANG Peng-wei | Tianjin University of TCM, Tianjin 300193, China | | | ZHANG Jin-bao | Tianjin University of TCM, Tianjin 300193, China | | | ZHANG Feng-qi | Tianjin University of TCM, Tianjin 300193, China | | | ZHANG Yan-jun | Tianjin University of TCM, Tianjin 300193, China | zyjsunye@163.com |
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| Abstract
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| [Objective] To observe the effects of D-galactose's dose and sex of mice on the aging model. [Methods] ICR mice with different gender were randomly divided into normal control group, low dose of D-galactose (100 mg/kg) group, middle dose(300 mg/kg) group, high dose(1 000 mg/kg). D-galactose was injected subcutaneously into the back of the neck daily and control group was injected with same amount of normal saline continuously for 10 weeks. Morris water maze was taken to observe the learning and memory ability. Blood sample was obtained to test the growth factors level and related parameters of oxidation. Brain tissue was harvested to observe the pathological morphological changes after HE staining. [Results] Compared with normal group, different dose of D-galactose could damage the learning and memory ability of mouse in different degrees, but no significant difference was observed between male and female mouse. The high dose group significantly extend the average latent period(P<0.05); Serum VEGF and IGF-1 in middle and high dose group were lower than control group with varying degrees (P<0.05 or P<0.01), No significant difference was observed between male and female; MDA concentrations of middle and high dose group were increased significantly when compared with normal group (P<0.05); SOD activity was significantly lower than normal group (P<0.05 or P<0.01). [Conclusion] ICR mouse subcutaneously received 300~1 000 mg/kg of D-galactose for 10 weeks could continuously decrease the learning and memory ability with different degrees obviously. This may be related to the increasing of oxidizing reaction and the decreasing of growth factor in serum. The mouse' gender had no obvious effect on aging model induced by D-galactose. |
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