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| Comparative study of different detection methods on prescription design of ginkgo biloba extract with sustained release preparation |
| Hits 2281 Download times 1777 Received:May 26, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2013.03.10 |
| Key Words
Ginkgo biloba extract;UV spectrophotometry;HPLC;sustained release preparation;similarity factor method |
| Author Name | Affiliation | E-mail | | GUO Ying-xin | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China | | | PAN Wei-san | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China | | | LI Fei | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China | | | ZHAO Qian-qian | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China | | | XIAO Wei | Jiangsu Kanion Pharmaceut Co Ltd, Lianyungang 222001, China
State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China | | | XU Lu | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China | | | YANG Xing-gang | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China | yangxg123@163.com |
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| Abstract
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| [Objective] To investigate the quantitative determination of flavonol glycosides in ginkgo biloba extract using UV spectrophotometry and HPLC, then establish a simple, effective and reliable method for the prescription design of ginkgo biloba extract of sustained release preparation. [Methods] The total flavonoid glycosides were used as the reference compound for UV spectrophotometry. The Quercetin, Kaempferide and Isorhamnetin were used as the reference compound for HPLC. The similarity between UV and HPLC was estimated by the similarity factor method, which was recommended by FDA. [Results] The methodological evaluation showed that those two detection methods met the requirements, and had good similarity. [Conclusion] During the course of prescription design of ginkgo biloba extract of sustained release preparation, the method of UV can be used to determine the content of flavonol glycosides instead of HPLC. This method is simple, accurate and reliable. |
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