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| Quantitative determination of 1-Deoxynojirimycin in San-Ye tablets by HPLC-MS/MS |
| Hits 2402 Download times 2067 Received:April 26, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2013.03.11 |
| Key Words
San-Ye tablets;1-Deoxynojirimycin;HPLC-MS/MS;quantitative determination |
| Author Name | Affiliation | E-mail | | JIANG Jia | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae (Tianjin University of Traditional Chinese Medicine) Ministry of Education, Tianjin 300193, China | | | LIU Hong | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae (Tianjin University of Traditional Chinese Medicine) Ministry of Education, Tianjin 300193, China | tjzylh@yahoo.com | | LI Xue-ru | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae (Tianjin University of Traditional Chinese Medicine) Ministry of Education, Tianjin 300193, China | | | HAO Yu | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae (Tianjin University of Traditional Chinese Medicine) Ministry of Education, Tianjin 300193, China | | | ZHANG De-qin | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae (Tianjin University of Traditional Chinese Medicine) Ministry of Education, Tianjin 300193, China | |
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| Abstract
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| [Objective] To develop a HPLC-MS/MS method for determination of 1-Deoxynojirimycin (DNJ) in San-Ye tablets. [Methods] The column was GL Sciences Inc. NH2 (150 mm×4.6 mm, 5 μm), the mobile phase was acetonitrile (cantain 0.1% formic acid)-0.1% formic acid (80:20,V:V), the flow rate was 0.5 mL/min; MS detector: Electrospray ionization (ESI) sources was applied and operated in the position ion mode. Multiple reaction monitoring (MRM) modes with the transition of 164.1/110.2 was used to quantify DNJ. [Results] The linear range of DNJ was 61～2 440 ng/mL (r=1.000, n=6). The lowest limit of detection was 4.26 ng/mL. The average recovery rate was 105.5%, RSD was 7.9%. [Conclusion] The method is simple, rapid, accurate, and can be applied to the determination of DNJ in San-Ye tablets. |
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