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| Determination of total saponins from Semen Ziziphi Spinosae |
| Hits 2798 Download times 1675 Received:July 11, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2013.04.11 |
| Key Words
Semen Ziziphi Spinosae;total saponins;content determination |
| Author Name | Affiliation | | ZHAO Qi-duo | Tianjin University of TCM, Tianjin 300193, China | | SHU Le-xin | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To determine the content of the total saponins from Semen Ziziphi Spinosae with purificated by macroporous resin.[Methods] The total saponins from semen Ziziphi spinosae were obtained by ethanol extraction, butanol extraction and macroporous resin purification. With ginsenoside Rb1 as standard, vanillin（sulfuric acid） as chromogenic agent, the ultraviolet spectrophotometry absorption wavelength was 543nm. [Results] The standard curve was linear in the range of 1.818 ~29.09 mg/L. The regression equation was Ŷ= 32.526X-1.675 9, r=0.999 7. The RSD of precision and recurrency were 0.09% and 0.28%, the RSD of control substance and testing substance in stability test were 0.42% and 1.13%, the average recoveries was 99.88%, RSD was 1.13%. The average content of total saponin in samples was 54.59%, RSD was 0.38.[Conclusion] The content determination method is feasible, stable, and can be used for quality control of extract. |
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