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| In vitro evaluation and cellular biocompatibility of Scutellarein-loaded PLGA nanoparticle |
| Hits 2356 Download times 2312 Received:September 20, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2014.01.10 |
| Key Words
controlled release;PLGA nanoparticles;Scutellarein;biocompatibility |
| Author Name | Affiliation | E-mail | | LI Nan | Tianjin State Key Laboratory of Modern Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin Univesity of TCM, Tianjin 300193, China | | | LIU Zhi-dong | Tianjin State Key Laboratory of Modern Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin Univesity of TCM, Tianjin 300193, China | | | FAN Li-li | Tianjin State Key Laboratory of Modern Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin Univesity of TCM, Tianjin 300193, China | | | LI Lin | Tianjin State Key Laboratory of Modern Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin Univesity of TCM, Tianjin 300193, China | | | WANG Ai-chao | Tianjin State Key Laboratory of Modern Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin Univesity of TCM, Tianjin 300193, China | | | GUO Li-li | Tianjin State Key Laboratory of Modern Chinese Medicine, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin Univesity of TCM, Tianjin 300193, China | | | PAN Wei-san | Shenyang Phanmaceatieal University, Shenyang 110016, China | ppwwss@163.com |
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| Abstract
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| [Objective] To prepare Scutellarein loaded PLGA nanoparticles and characterize the physicochemical properties, in vitro release behavior and biocompatibility of HCEpic cells. [Methods] The PLGA nanoparticles were prepared by emulsion solvent diffusion method. The characterization and release effect of PLGA NPs in vitro were evaluated. The main outcome measures included size, Zeta potential, Encapsulation efficiency, the drug content of the final nanoparticles etc. The in vitro release curve was drawn. The cytotoxicity of PLGA NPs were evaluated by cell proliferation assay. [Results] The particle sizes, Zeta potential, Encapsulation efficiency and the drug content of the final nanoparticles were 128.4 nm, -21.1 mV, (92.5±2.35)% and (23.12±1.45)%, respectively. The release of Scutellarein from PLGA nanoparticles were markedly reduced compared with Scutellarein solution. Cell proliferation assay revealed the PLGA-NPs did not damage the cell growth in vitro, indicating a good compatibility. [Conclusion] The PLGA-NPs prepared by emulsion solvent diffusion method have a good release effect and good biocompatibility in vitro. |
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