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| Role and mechanism of macrophages and its related-factors in vulnerable plaque of rabbits |
| Hits 1936 Download times 1791 Received:December 08, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2014.02.05 |
| Key Words
balloon injury;high-fat diet;vulnerable plaque;inflammatory reaction |
| Author Name | Affiliation | E-mail | | HU Xian-tong | Pathology Laboratory of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Dong-ming | Department of Experimental Teaching of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GAO Qing | Pathology Laboratory of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Hong-xia | Department of Experimental Teaching of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Yi-jing | Pathology Laboratory of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Ying-chang | Pathology Laboratory of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Xi-juan | Department of Experimental Teaching of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | xijuanjiang@foxmail.com |
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| Abstract
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| [Objective] To investigate the role and the mechanism of macrophages and their related factors in the vulnerable plaque of rabbit. [Methods] Rabbits were fed with high-fat diet for two weeks. The rabbits were divided into two groups: the sham group and the model group. The model group received a balloon injury in its aorta abdominalis. The arteria cruralisin the sham group was exposed but the balloon was not inserted. The rabbits underwent a pharmacological triggering with Chinese Russell's viper venom and histamine at the end of 10th week after the surgery these animals were then killed. The ruptured plaque was counted. The percentage of macrophage in the plaque was calculated by immunohistochemical staining. The inflammatory factors, such as CRP, MCP-1 and TNF-α were detected by Elisa. [Results] Compared with sham group, in the model group there were significantly more ruptured plaque, larger plaque area, bigger lipid core, thinner fibrous cap, more foam cells and inflammatory cells. The percentage of macrophages in the plaque of model group was higher than that of sham group(P<0.01). Compared with the sham group, the inflammatory factors, such as CRP, MCP-1 and TNF-α in the model group were up-regulated evidently. [Conclusion] Balloon injury of endothelial cells can promote the development of atherosclerosis. The inflammatory reaction mediated by macrophages may be a important mechanism in the formation of vulnerable plaque. |
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