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| Protective effect of Danshen injection in high concentration on endothelial cells injured by high glucose |
| Hits 2377 Download times 2147 Received:November 17, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2014.02.06 |
| Key Words
Danshen injection;endothelial cell;protection |
| Author Name | Affiliation | | WANG Zhi-hua | Department of Paediatrics, Nankai Hospital, Tianjin 300100, China | | Wang Yu-shui | Department of Paediatrics, Nankai Hospital, Tianjin 300100, China |
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| Abstract
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| [Objective] To explore the effect of Danshen injection on human vascular endothelial cells injured by high glucose. [Methods] The endothelial cells were obtained by perfusion digestion of blood from umbilical vein using type Ⅰ collagenase (0.1%), and the second passage of endothelial cells was incubated in 96 plate. The formed subconfluent, endothelial cells were incubated with different medium respectively: normal control, group inured with high glucose and protected group with different concentration of Danshen injection. 48 hours later, the number of survived cells were determined with MTT. [Results] At the concentration of 10-1, 10-2, 10-3 mg/ml, Danshen injection could improve the protective effect against the injury of endothelial cells harmed by high glucose(P<0.05), at the concentration of 10-4 mg/ml could not improve the protective effect (P>0.05). [Conclusion] Danshen injection of high concentration can protect endothelial cells harmed by high glucose. |
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