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| The identification of cryptic taxonomic diversity among TCM Eupolyphaga sinensis based on the sequences of DNA barcoding region |
| Hits 2456 Download times 1721 Received:February 15, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.02.07 |
| Key Words
Eupolyphaga sinensis;DNA Barcoding;Cryptic Species;Genetic Analysis |
| Author Name | Affiliation | E-mail | | TIAN Xiao-xuan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Jie | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | XIAO Mi | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Peing | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHU Yan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | yanzhu.harvard@gmail.com |
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| Abstract
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| [Objective] To investigate the cryptic species diversity among Eupolyphaga sinensis as one main resource of TCM Tubiechong, based on the DNA Barcoding COI sequences. [Methods] Mitochondrial COI fragments from 33 individuals of 8 batchs of Eupolyphaga sinensis were amplified and sequenced. The haplotypes of COI and then the variation locuses were analysed. The phylogenetic trees based on NJ and MP methods were constructed, and the cryptic species were discovered by the genetic distance analysis of intra-population and inter-provisional species samples. [Results] Provisional species EsPS1 and EsPS2 were distinguished by phylogenetic analysis. The species screening threshold SST of COI fragments was set as 5.33%, and the minimal genetic distance between EsPS1 and EsPS2 was 12.00%, which was higher than SST significantly.[Conclusion] There is cryptic taxonomic diversity among TCM Eupolyphaga sinensis. |
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