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| Quantitative analysis of two Iridoids in Figwort Roots by QAMS method |
| Hits 1983 Download times 2642 Received:November 11, 2013 |
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| DOI
10.11656/j.issn.1673-9043.2014.02.10 |
| Key Words
QAMS;figwort roots;relative correction factor;HPLC |
| Author Name | Affiliation | | BIAN Xiao-xia | Medical College of Tianjin, Tianjin, 300222, China | | LUO Yue-e | Medical College of Tianjin, Tianjin, 300222, China | | YAN Li-hua | Institule of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract
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| [Objective] To develop a content determination method for simultaneous determination of harpagide and harpagoside in figwort roots, and establish its methodology. [Methods] The HPLC method is adopted to assay harpagide glycosides as internal standard, establishe the relative correction factor with harpagoside. Five batches figwort roots are determined the two iridoids content using, quantitative analysis multicomponents by single marker(QAMS) and compared with the external standard method. [Results] No significant differences was found in the quantitative analysis results of the two iridoids content between external standard method and QAMS method.[Conclusion] The use of relative correction factor is feasible to assay the content of the harpagoside. QAMS method can be used for the quality assessment of figwort roots. |
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