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| Effect of Tongluo Jiunao injection on expression of placental growth factor of brain microvascular endothelial cells in rats injured by oxygen-glucose deprivation |
| Hits 1896 Download times 1742 Received:February 18, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.03.05 |
| Key Words
brain microvascular endothelial cells;oxygen-glucose deprivation;placental growth factor;vascular endothelial growth factor receptor-1;Tongluo Jiunao injection |
| Author Name | Affiliation | | DU Huan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | GAO Qing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract
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| [Objective] To observe the effects of Tongluo Jiunao injection on the expression of the placental growth factor (PLGF) and the vascular endothelial growth factor receptor-1(VEGFR-1) in brain microvascular endothelial cells of rats injured by oxygen-glucose deprivation.[Methods] The model of oxygen-glucose deprivation (OGD) was established in the primarily cultured brain microvascular endothelial cells. The endothelial cells were divided into three groups: normal control group, OGD model group and Tongluo Jiunao injection-treated group. The PLGF content of endothelial cells in conditioned media was tested by ELISA. The expression of VEGFR-1 protein in endothelial cells were measured by western blotting. [Results] The expressions of PLGF content and VEGFR-1 were significantly higher after treating with Tongluo Jiunao injection when compared with the OGD model group (P<0.01). [Conclusion] This traditional Chinese medicinal preparation can enhance the expression of PLGF and VEGFR-1 of endothelial cells in rats injured with OGD, suggesting the therapeutic potential of Tongluo Jiunao injection for ischemic cerebral vascular disease. |
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