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| Effects of Cordyceps sinensis on the migration and invasion capability of human gastric adenocarcinoma cell line MKN-28 |
| Hits 1947 Download times 1747 Received:March 25, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.03.07 |
| Key Words
Cordyceps sinensis;gastric adenocarcinoma;MKN-28 cell line;Ezrin;MMP9 |
| Author Name | Affiliation | E-mail | | SUN Guang-bin | Department of Gastroenterology, the Fifth Central Hospital of Tianjin, Tianjin 300450, China | | | LAN Lan | Department of Integrated Traditional and Western Medicine, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, China | lanlan9427@126.com | | TANG Hong-mei | College of Life Science, Hebei United University, Tangshan 063000, China | | | LI Ning | Department of Immunology, School of Medicine, Nankai University, Tianjin 300071, China | | | WANG Yue | Department of Immunology, School of Medicine, Nankai University, Tianjin 300071, China | |
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| Abstract
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| [Objective] To explore the effects of Cordyceps sinensis on the migration and invasion capability and on the expression of Ezrin and MMP9 in human gastric adenocarcinoma cell line MKN-28. [Method] The inhibitory effects of Cordyceps sinensis on the proliferation of MKN-28 cell line were evaluated by CCK-8 assay. The effects of Cordyceps sinensis on the migration and invasion ability of the cells were observed through scratch assay and Boyden chamber, respectively. RT-PCR system detecting the mRNA gene expression changes of Ezrin and MMP9 in MKN-28 cell line. [Result] Cordyceps sinensis from 200 μg/mL to 800 μg/mL can obviously inhibited the proliferation of the human gastric adenocarcinoma cell line MKN-28. Scratch and matrigel invasion experiments showed that cordyceps sinensis significantly inhibited cell migration and invasion. Cordyceps sinensis inhibited the gene expression of Ezrin and MMP9 in MKN-28 cell line. [Conclusion] Cordyceps sinensis may inhibited cell proliferation, migration and invasion through down regulating the expression of Ezrin and MMP9, then suppressing the tumor growth. |
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