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| Carboxymethyl modification and antioxidant activity of sophora alopecuroides polysaccharide |
| Hits 2299 Download times 2201 Received:January 14, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.03.08 |
| Key Words
Sophora alopecuroides polysaccharide;carboxymethylation;substitution degree;orthogonal test;antioxidation activity |
| Author Name | Affiliation | E-mail | | SHEN Lin-hui | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Institute of Medical Pharmaceutical Sciences, Tianjin 300020, China | | | LIU Li-xia | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Institute of Medical Pharmaceutical Sciences, Tianjin 300020, China | | | CHEN Guan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Institute of Medical Pharmaceutical Sciences, Tianjin 300020, China | | | ZHAO Yong-an | Tianjin Medical University, Tianjin 300070, China
Tianjin Institute of Medical Pharmaceutical Sciences, Tianjin 300020, China | | | TAO Zun-wei | Tianjin Institute of Medical Pharmaceutical Sciences, Tianjin 300020, China | taozunwei@yahoo.com.cn |
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| Abstract
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| [Objective] Carboxymethylated sophora alopecuroides polysaccharide was prepared by sophora alopecuroides polysaccharide as the raw material. [Methods] The method was optimized by L9(34) orthogonal test with the substitution degree of carboxymethylation as the index. [Results] The results showed that the optimized condition 50 mg of Sophora alopecuroides polysaccharide was reacted with 0.5 g of Sodium chloroacetate,with reaction temperature of 55℃, reaction time of 5 h, 25% of sodiumhydroxide concentration. The maximum substitution degree (DS) was 1.50 at least. [Conclusion] The preparation process is effective. Meanwhile, carboxymethylated sophora alopecuroides polysaccharide has more accelerated scavenging capacities against hydroxyl, which is much better than that before carboxymethylation. |
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