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| Preventive effect of two ophthalmic preparations of baicalin against seleniumcataract |
| Hits 1802 Download times 1744 Received:February 05, 2014 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2014.03.10 |
| Key Words
seleniumcataract;baicalin;SOD;GSH;Western blotting |
| Author Name | Affiliation | E-mail | | HAN Zhen-zhen | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Nan | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | linan20080402@163.com | | GUO Li-li | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | TONG Ling | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHEN Jing | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To study the anti-selenite cataract effect of two ophthalmic preparations of Baicalin, Baicalin-loaded solid lipid nanoparticles (BA-SLNs) and Baicalin-loaded solid lipid nanoparticles in gel (BA-SLNsG). [Methods] The selenite cataract model was reproduced on Wistar rats of 12-days old. 20 μL of two ophthalmic solutions/eye drops were given to the treatment groups (TP), meanwhile same volume of saline was given to blank group (BP) and model group (MP), same volume of pirenoxine sodiumwas given to control group (CP). Drugs were delivered 4 times a day for 8 days. The change of lens was observed daily. The expression of α A-Crystallin was tested by Western blotting. The antioxidant activity was determined by superoxide disproportionation enzymes and glutathione. [Results] The degree of lens opacity of a TP was significantly lighter than that of MP. SOD vitality of in BP and TP were significantly higher than MP (P<0.01);GSH content of BP and control group were significantly higher than MP (P<0.01). BA-SLNs group and BA-SLNsG group were higher than model group(P<0.05). The expression of α A-Crystallin in model group was higher than others. [Conclusion] Both BA-SLNs and BA-SLNsG can improve the antioxidant capacity of crystalline lens by regulating down α A-Crystallin expression. It shows that the possibility of the application of BA-SLNs and BA-SLNsG into clinical. |
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