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| Effect of Heying Anxin prescription on oxidative stress injury in embryo myocardium of rats |
| Hits 1798 Download times 1565 Received:March 14, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.04.07 |
| Key Words
Heying Anxin prescription;H2O2;myocardium cell;oxidative stress |
| Author Name | Affiliation | E-mail | | HE Wei | Hebei North University, Zhangjiakou 075029, China | | | LI Yong-min | Hebei North University, Zhangjiakou 075029, China | liyongmin2001@sina.com | | ZHANG Hai-xia | Hebei North University, Zhangjiakou 075029, China | | | ZHU Juan | Hebei North University, Zhangjiakou 075029, China | | | GAO Yue | Hebei North University, Zhangjiakou 075029, China | |
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| Abstract
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| [Objective] To investigate the effect of Heying Anxin prescription on embryo myocardium injury caused by H2O2.[Methods] Reflux condenser extraction with 70% ethanol of Heying Anxin prescription was used and the cardiomyocytes were incubated with H2O2(200 μmol/L) for 2 hours in model group. the same dose of H2O2 was administered for 2 hours after treatment with Heying Anxin prescription (500 mg/kg, 250 mg/kg, 125 mg/kg) in therapeutic group and incubated for another 24 hours. Metabolic rate of MTT, content of MDA, activity of LDH and the levels of CAT and SOD in each group were measured.[Results] Metabolic rate of MTT: compared with control group, metabolic rates of MTT was decreased significantly in H2O2 group(P<0.01), while Heying Anxin prescription had no significant effect on cardiac myocytes of MTT metabolic rates. Content of MDA: compared with that of H2O2 group, the content of MDA was decreased in Heying Anxin prescription groups significantly(P>0.05).Content of LDH: compared with that of control group, the content of LDH was decreased in Heying Anxin prescription groups significantly(P>0.05).Content of CAT: compared with that of H2O2 group, the content of CAT was elevated in Heying Anxin prescription groups significantly(P>0.05).Content of SOD:compared with that of H2O2 group,the content of SOD was elevated in Heying Anxin prescription groups significantly(P<0.01).[Conclusion] Heying Anxin prescription has significant protective effects on injured myocardium cell induced by H2O2 in rats, and it may be related to the inhibition of oxidative damage. |
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