|
| Establishment of rapid callus proliferation culture system and evaluating content of secondary metabolite in Herba Artemisiae Annuae |
| Hits 1766 Download times 1428 Received:February 15, 2014 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2014.04.09 |
| Key Words
Artemisia annua;tissue culture;quininic acid;coumarin compounds;secondary metabolites |
| Author Name | Affiliation | E-mail | | GUO Xin-rong | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Lin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Zhen-zhong | Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang 222001, China | | | XIAO Wei | Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang 222001, China | | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang 222001, China | xiaowei@163.com |
|
| Abstract
|
| [Objective] To establish the rapid callus proliferation culture system of Herba Artemisiae Annuae and determine the content of phenylpropionic acids and coumarins.[Methods] The inflorescence, leaf and petiole were cut down and selected as explant and induced into callus under sterile conditions. Then, the callus was planted in MS with different combinations of different hormones. Finally, the content of phenylpropionic acid and coumarins was determined.[Results] The rapid callus proliferation culture system of Herba Artemisiae Annuae was established successfully by tissue culture. Kinetics of growth accumulation and the best harvest of phenylpropionic acid and coumarins biosynthesis were evaluated.[Conclusion] Kinetics of growth accumulation of secondary metabolites of Artemisia Annue can be successfully established by tissue culture. These methods provide a new way to explore the variation of the secondary metabolites in culture system optimization and industrial scale production of Artemisia Annua. |
|
|
|
|
|
