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| Biological characteristics of angiogenesis of microvascular endothelial cells in rat with myocardial ischemia |
| Hits 1945 Download times 1601 Received:April 26, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.05.07 |
| Key Words
ischemic myocardium;microvascular endothelial cell;angiogenesis;biological characteristics |
| Author Name | Affiliation | | DAI Guo-hua | Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China | | SONG Xian-bo | Shandong University of Traditional Chinese Medicine, Jinan 250014, China | | MA Pei-ze | Shandong University of Traditional Chinese Medicine, Jinan 250014, China | | LIU Ning | Shandong University of Traditional Chinese Medicine, Jinan 250014, China | | YAO Jing | Shandong University of Traditional Chinese Medicine, Jinan 250014, China |
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| Abstract
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| [Objective] To observe the biological characteristics of angiogenesis of cardiac microvascular endothelial cells in rat with myocardial ischemia. [Methods] The model of myocardial ischemia was established with ligation method. The cardiac microvascular endothelial cells (CMECs)were cultured by the method of planting myocardium tissue. The morphology of CMECs were observed by inverted phase contrast microscope. Its specific antigen was observed by immunocytochemistry. CMECs of ischemic rats was served as ischemia group (group I), CMECs of normal rats was served as control group (group N); MTT were used to draw the cell growth curve and detect the proliferation. The cell migration ability were measured by wound healing assay, tube-like structure formation were observed by inverted phase contrast microscope; Real-time PCR were used to detect the mRNA expression of ERK and p53.[Results] The CMECs of rats had the typical characteristics of microvascular endothelial cells, factor Ⅷ,CD31 related antigen were all positive by immunocytochemical stain identification. The transfer window period were the first day, and the tube formation window period were the second day and the proliferation window period were respectively the third day, and the sixth day of group N and I. When compared two groups, rate of migration, proliferation and tube formation in the group I were lower than group N. The most obvious reducing was migration rate (P<0.01). The mRNA expression of ERK and p53 in the group I were higher than that in group N(P<0.05).[Conclusion] The angiogenesis capacity of ischemic CMECs is decreased obviously. The angiogenesis proliferation window period is changed, and this may be related to the change of mRNA expression of ERK and p53. This can provide some experimental basis for the further study of genomics and drug intervention. |
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