|
| Effect of electroacupuncture on cerebral blood flow and secretion of ET-1 in rats with ischemic stroke sequelae |
| Hits 1660 Download times 1651 Received:May 12, 2014 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2014.05.09 |
| Key Words
electroacupuncture;ischemic stroke sequelae;cerebral blood flow;ET-1 |
| Author Name | Affiliation | E-mail | | WANG Juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Jian | Institute of Acupuncture, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | yooken@sina.com |
|
| Abstract
|
| [Objective] To observe the effect of electroacupuncture on cerebral blood flow and secretion of ET-1 in rats with ischemic stroke sequelae.[Methods] Thirty healthy adult male Wistar rats were randomly divided into electroacupuncture group, model group and sham operation group with 10 rats in each group. Referencing the Zea-Longa line tied method the model of middle cerebral artery occlusion (MCAO)was reproduced. Five weeks later the model of ischemic stroke sequelae was considered to be formed. Rats in electroacupuncture groups were punctured on "Baihui","Shuigou". Two weeks later, the Laser Doppler flow-meter was used to monitor the cerebral blood flow of rats and immunohistochemical method was used for determination of the level of endothelin-1 (ET-1)in brain tissue. [Results] Compared with the sham operation group, the cerebral blood flow was reduced in the model group (P<0.05). Compared with model group, the cerebral blood flow was significantly increased in the EA group (P<0.05). In the model group the total area with ET-1 protein expression-positive substance was higher than that in sham group (P<0.05). The total area of the positive cells of ET-1 protein expression in the brain tissue of EA group had a lower trend than that in the model group. But there was no significant difference between these two groups (P>0.05).[Conclusion] After cerebral ischemia, cerebral tissue ET-1 levels has increased significantly. EA can inhibit the up-regulation of ET-1 and improve the blood flow of the rats with ischemic stroke sequelae. |
|
