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| Influence of Jianpi Yifei II decoction on inflammatory cytokines and metalloproteases in lung tissues of rats induced by cigarette smoke and LPS |
| Hits 1670 Download times 2405 Received:August 14, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.06.07 |
| Key Words
Jianpi Yifei II;metalloprotease;inflammatory cytokines;rat |
| Author Name | Affiliation | | LIN Lin | The second Affliliated Hospital of Guangzhou University of Tradittonal Chinese Medicine, Guangzhou 510120, China | | XU Yin-ji | The second Affliliated Hospital of Guangzhou University of Tradittonal Chinese Medicine, Guangzhou 510120, China | | WU Lei | The second Affliliated Hospital of Guangzhou University of Tradittonal Chinese Medicine, Guangzhou 510120, China | | CHEN Zhi-xi | Guangzhou University of Chinese Medicine, Guangzhou 510405 | | YU Xu-hua | The second Affliliated Hospital of Guangzhou University of Tradittonal Chinese Medicine, Guangzhou 510120, China |
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| Abstract
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| [Objective] To investigate the influence of Jianpi Yifei II on inflammatory cytokines and and metalloproteases in lung tissues of rats induced by cigarette smoke combined LPS and to provide theoretical basis to doctors for clinical application.[Methods] The rat model of lung injury was established by using intratracheal injection of LPS combining cigarette smoke. All rats were randomly divided into normal group, model group, Jianpi Yifei II low does group, Jianpi Yifei high does group and prednisone group. General condition of rats was observed and recorded first, then we detected the expression of MMP-9 and TIMP-1 and contents of TGF-β1,TNF-α and IL-8 in lung tissue homogenate or plasma by special assay kits. [Results] After inducing lung injury by treating rats with LPS and cigarette smoke, the general condition of rats in Jianpi Yifei II group and prednisone group was better than in model group. And the ratio of MMP-9/TIMP-1 was lower in the Jianpi Yifei Group and prednisone group than in model group(P<0.05 or P<0.01).In addition, compared to model group, the levels of TNF-α,IL-8 and TGF-β1 in lung tissues or plasma were significantly decreased in Jianpi Yifei II group and prednisone group(P<0.05 or P<0.01). [Conclusion] Jianpi Yifei II could protect lung tissue from the cigarette smoke and LPS by regulating levels of inflammatory cytokines and MMP-9/TIMP-1 ratio. |
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