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| Effects of bakuchiol on secretion of testosterone and estradiol from H295R cells in human with carcinoma of adrenal cortex |
| Hits 1970 Download times 1777 Received:July 10, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.06.08 |
| Key Words
bakuchiol;H295R cell;testosterone;estradiol |
| Author Name | Affiliation | E-mail | | MAO Hao-ping | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianｊin 300193, China | haoping_mao@126.com | | NIU Zi-chang | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Xing-ye | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianｊin 300193, China | | | QIU Yan-rong | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianｊin 300193, China | |
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| Abstract
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| [Objective] To study the effects of bakuchiol on testosterone and estradiol secretion from H295R cells. [Methods] The researches were divided into control group and bakuchiol groups. The effects of bakuchiol on cellular activites and the secretion of testosterone and estradiol were detected by CCK-8 method and ELISA method. The levels of CYP17A1 and CYP19A1 mRNA were also detected by Real-time PCR.[Results] Bakuchiol could not affect CYP17A1 mRNA leverl but could up-regulate CYP19A1 mRNA. Bakuchiol had the effect of decreasing the secretion of testosterone but increasing the secretion of estradiol. [Conclusion] Bakuchiol can stimulate estradiol secretion from H295R cells. |
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