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| Influence of autophagy on nerve regeneration induced by electric acupuncture treatment in rats with experimental sciatic nerve injury |
| Hits 1745 Download times 1677 Received:August 15, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.06.09 |
| Key Words
autophagy;electric acupuncture;nerve regeneration;sciatic nerve function index;histology |
| Author Name | Affiliation | E-mail | | ZHAO Wei | School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Yan-xiang | School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Zhan-kui | School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Rui | School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GUO Yi | School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | guoyi_168@163.com |
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| Abstract
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| [Objective] To study the role of autophagy in electric acupuncture treatment of peripheral nerve regeneration.[Methods] The experimental clamped injury of sciatic nerve was induced in rats through operation. Intervention was given with electric acupuncture treatment,autophagy inhibitor 3-MA. After that treatment at different time points the functional indexes of sciatic nerve (SFI) were determined and the histology of regeneration of nerve was observed.[Results] The comparison between groups found that the SFI in electric acupuncture combined with autophagy inhibitor was more less than other groups (P<0.05). The comparison of inter-groups revealed that the SFI in model group and electric acupuncture group at the first, second and seventh day was not significantly different. All of them were become different at twenty-eight day(P<0.01). The level of SFI in electric acupuncture combined with autophagy inhibitor was not different at the first and second day but at the 7th and 28th day become significantly different(P<0.05) and was less than the above groups. In addition,the biopsy of regenerative nerve tissue stained by HE and silver was showed that the continuity and intensity of regenerative nerve tissue and nerve fibers of electric acupuncture + autophagy inhibitor group was inferior to other groups on 28th day.[Conclusion] The electric acupuncture plays a important role in the course of long-term recovery of sciatic nerve functions. Under the same condition of electric acupuncture,the inhibition of autophagy will produce a larger negative effect for the functional recovery and structural improvement of sciatic nerve, suggesting that the functional recovery and structural improvement can be promoted through cellular autophagy. |
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