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| Simultaneous determination of ephedrine and pseudoephedrine in rat' plasma and their pharmacokinetics determined by LC-MS/MS |
| Hits 1998 Download times 1492 Received:August 14, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.06.10 |
| Key Words
ephedrine;pseudoephedrine;pharmacokinetic;Sanao decoction;LC-MS/MS |
| Author Name | Affiliation | E-mail | | DOU Ting | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | OUYANG Hui-zi | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Xing-Rui | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | BO Fang | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | hejun673@163.com |
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| Abstract
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| [Objective] To develop a LC-MS/MS method for the determination of ephedrine and pseudoephedrine in rat's plasma simultaneously and to study the pharmacokinetics of ephedrine and pseudoephedrine of rat's plasma after oral administration of SD(Sanao Decoction).[Methods] The blood plasma samples were extracted through alkali and ethyl acetate liquid-liquid. Phenylalanine hydrochloride was used as the internal standard. Separation was performed using an Agilent Zorbax SB-C18(4.6 mm×150 mm, 5 μm). The analytical column was acetonitrile-0.1% formic acid in water solution (4:96). Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 166.2→m/z 148.2, m/z 166.2→m/z 148.2 and m/z 152.1→m/z 134.1 were used to quantify ephedrine, pseudoephedrine and phenylalanine hydrochloride, respectively. [Results] The linearity ranged from 20 to 10 000 μg/L. RSD for inter-match and intra-match were not more than 5.3% and the average extracted recovery for all the high, middle and low concentration was more than 64.7%. [Conclusion] The analytical method is proved to be special, sensitive, rapid and suitable for the pharmacokinetic study of ephedrine and pseudoephedrine. |
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