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| Effect of Sanjia powder on expression of protein of α-SMA, TIMP-1, ROCK-Ⅰ in hepatic stellate cell |
| Hits 1849 Download times 1478 Received:August 16, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.06.11 |
| Key Words
Sanjia powder;α-SMA;TIMP-1;ROCK-Ⅰ;protein expression |
| Author Name | Affiliation | | ZHENG Xu-rui | Shanxi University of Traditional Chinese Medicine, Xianyang 712046, China | | HUI Yi | Shanxi University of Traditional Chinese Medicine, Xianyang 712046, China | | YAN Shu-guang | Shanxi University of Traditional Chinese Medicine, Xianyang 712046, China | | SONG Xiao | Shanxi University of Traditional Chinese Medicine, Xianyang 712046, China | | CAO Ning | Shanxi University of Traditional Chinese Medicine, Xianyang 712046, China | | WANG Li-feng | Shanxi University of Traditional Chinese Medicine, Xianyang 712046, China | | CHAO Xu | Shanxi University of Traditional Chinese Medicine, Xianyang 712046, China |
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| Abstract
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| [Objective] The expression of α-SMA, TIMP-1 and ROCKⅠ in the hepatic(HSC-T6) stellate cell were measured to study the proliferating effect of Sanjia powder. [Methods] HSC-T6 cells interfered by Sanjia powder-containing serum, RT-PCR and Western blot were used to detect the expression of α-SMA, TIMP-1, ROCKⅠ in the hepatic stellate cell. [Results] Real-time PCR and Western blot assay showed that Sanjia powder and Y-27632 could reduce the expression of ROCK-Ⅰprotein, TIMP-1 and a-SMA mRNA in HSC-T6 cells. [Conclusion] Sanjia powder can decrease the expression of ROCK-Ⅰ protein, TIMP-1 and a - SMA in HSC-T6 cells. |
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