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| Determination of chlorogenic acid,p-coumarin and hyperin of Cuscuta chinensis Lam. by high performance liguid chromatography |
| Hits 2495 Download times 2236 Received:August 17, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2014.06.15 |
| Key Words
Cuscuta chinensis Lam.;HPLC;Chlorogenic acid;P-coumarin;Hyperin |
| Author Name | Affiliation | E-mail | | TIAN Yu-fei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Jiao | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Jin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Lin | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Er-wei | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHANG Yan-xu | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | tcmcyx@126.com |
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| Abstract
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| [Objective] To establish a sensitive method for simultaneous determination of chlorogenic acid, p-coumarin and hyperin in order to provide some evidences for the quality control of Cuscuta chinensis Lam.. [Methods] Methanol and 0.05% formic acid were used as mobile phase. The sample was separated on an ODS C18 25 column(4.6 mm×250 mm,5 μm) by gradient elution. The flow rate was 1.0 mL/min. The wavelength was set at 360 nm. [Results] Chlorogenic acid, p-coumarin and hyperin were well separated by HPLC. Linearity ranges were 2.00~100 μg/mL(r=0.999 3), 6.25~100 μg/mL (r=0.999 1) and 0.50~5 0 μg/mL(r=0.999 1) for chlorogenic acid, p-coumarin and hyperin, respectively.[Conclusion] The simple and feasible HPLC method was established to evaluate the quality of Cuscuta chinensis Lam.. |
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