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| Effects of Yiqi Huoxue prescription on myocardial energy metabolic with CHF rats after myocardial infarction |
| Hits 1844 Download times 1443 Received:September 20, 2014 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2015.01.07 |
| Key Words
Yiqi Huoxue prescription;chronic heart failure;PGC-1α;ATP;BNP |
| Author Name | Affiliation | E-mail | | LIU Xiu-na | Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China | | | ZHANG Yan | The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China | yanzhang1016@126.com | | LI Hai | The Second Chinese Medicine Hospital in Shenyang, Shenyang 110101, China | |
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| Abstract
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| [Objective] To study the effects of Yiqi Huoxue prescription on chronic heart failure(CHF) rats after myocardial infarction. [Methods] Twenty rats were selected from eighty healthy SD rats as the blank control group, and the rest was used to prepare CHF rat model by coronary artery ligation in combination with exhausted swimming and food reducing. By detecting the heart color doppler ultrasound and observing signs of changes in rats to determine whether building success. The successful molding rats were randomly divided into Chinese medicine group, Western medicine group and model group, blank control group(n=15) and model group(n=14) were given conventional volume distilled water by intragastric administration, 1 time a day, the Chinese medicine group(n=15) was given 9.2 g/(kg·d) crude drugs dose of Yiqi Huoxue prescription every day by intragastric administration, Western medicine group (n=14) was given 1.5 mg/(kg·d) crude drugs dose of Lisinopril every day by intragastric administration. After continuous administration of 28 days, fast and only drink water for 12 hours, getting myocardial tissue and plasma. The concentration of BNP was detected by ELISA, and the concentration of ATP was detected by colorimetric method, using Real-time fluorescent quantitative PCR and immune protein imprinting method to detect PGC-1α gene and protein expression. [Results] Compared with the blank control group, the mRNA expression and the protein expression of PGC-1α and the concentration of ATP were decreased, while the concentration of BNP was increased in model, Chinese medicine and Western medicine group, showing statistical difference (P<0.05). Compared with the model group, the mRNA expression and the protein expression of PGC-1α and the concentration of ATP were decreased, while the concentration of BNP was increased in Chinese medicine and Western medicine group, showing statistical difference (P<0.05). Compared Chinese medicine group with the Western medicine group, the mRNA expression and the protein expression of PGC-1α and the concentration of ATP and the concentration of BNP had no obvious difference, showing there was no statistically significant difference between the groups(P>0.05). [Conclusion] Yiqi Huoxue prescription can activate the PGC-1α factor which is related to myocardial energy metabolism, increase the capacity of mitochondria, in order to improve and correct heart failure. |
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