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| Determination of Baicalin and Linarin in Cuochuang Gel by UPLC |
| Hits 1833 Download times 1369 Received:November 07, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2015.01.12 |
| Key Words
Cuochuang Gel;baicalin;Linarin;UPLC;content determination |
| Author Name | Affiliation | E-mail | | FAN Li-li | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Nan | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GU Xing | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHU Yun | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Zhi-dong | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | lonerliuzd@163.com | | XIA Qing-mei | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GUO Li-li | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Ai-Chao | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To establish a UPLC method for content determination of Baicalin and Linarin in Cuochuang Gel. [Methods] The column was Acquity BEH C18(2.1 mm×50 mm, 1.7 μm). The mobile phase consisted of a mixture acetonitrile-1%formic acid(20:80). The flow rate was 0.3 mL/min.The detection wavelength was 320 nm under 35 ℃. [Results] The calibration curves for baicalin and Linarin were linear in the ranges of 3.767~75.34 μg and 0.840 96~33.638 4 μg, and the average recovery was 99.04% and 97.42%(n=6), with RSD of 1.97% and 1.81%, respectively. [Conclusion] This method was simple, precise and repeatable, which can be used for quality control of Cuochuang Gel. |
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