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| Regulated Pecam1 gene expression in bone marrow-derived Lin- cells by wogonin |
| Hits 1956 Download times 1713 Received:November 14, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2015.02.06 |
| Key Words
bone marrow-derived Lin- cells;wogonin;key functional gene;adhesion ability |
| Author Name | Affiliation | E-mail | | LI Hui-ying | Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | MA Ya-ke | Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | YANG Yue | Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | | | WANG Hong | Research Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China | wanghongsys@126.com |
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| Abstract
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| [Objective] To explore the effects of wogonin on the proliferation, differentiation, migration, adhesion gene expression of bone Marrow-derived Lin- cells. [Method] Using magnetic activated cell sorting (MACS) to separate Lin- cells from bone marrow and using CD117 as a marker for cell identification. To investigate the effect of wogonin on functional gene expression of bone marrow-derived Lin- cells by real-time-RT-PCR array technology. Adhesion method was used to detect the effect of wogonin on the adhesion of Lin- cells. [Result] Flow cytometry results showed that, mononuclear cells derived from bone marrow of mice after MACS, the separation rate of CD117 was 93.7%. The PCR chip results showed that the gene expression of platelet endothelial cell adhesion molecule -1 (Pecam1) of Lin-cell was significantly up-regulation by wogonin. The adhesion ability experimental result showed that wogonin could enhance the adhesion ability of Lin- cells. [Conclusion] It is an ideal separation method by using MACS to separate Lin- cells from bone marrow. Wogonin may up regulate Lin- cells in bone marrow of mice Pecam1 gene expression, thereby increasing the adhesion ability of Lin- cells. |
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