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| Preliminary mechanism study of disruption of chronobiological rhythm promoting diabetic nephropathy through “deficiency of Qi and Yin” pathogenesis: verification in animal experiments |
| Hits 1614 Download times 2509 Received:November 05, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2015.02.07 |
| Key Words
disruption of chronobiological rhythm;diabetic nephropathy;the ratio of cAMP and cGMP;deficiency of Qi and Yin;pathogenesis discussion |
| Author Name | Affiliation | E-mail | | CHEN Gang-yi | The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China | | | WEN Shu-ting | The First Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China | wsttingzi@163.com | | LIN Yu | The First Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China | | | LI Wen-qing | The First Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China | | | LI Ming-shun | The First Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China | | | WANG Jun-yue | The Second Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China | | | WEN Hui-hong | The First Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China | | | NING Wei-jie | The First Clinical Medical College of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China | |
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| Abstract
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| [Objective] To observe the effect of “deficiency of Qi and Yin” pathogenesis in chronobiological rhythm disordered diabetic nephropathy rats. [Methods] Sixty-four Male Sprague Dawley(SD) rats were randomly divided into normal control group, chronobiology disorder group, diabetic nephropathy(DN) group, and DN chronobiology disorder group(n=16 in each group). DN rats and chronobiology disorder DN rats were injected intraperitoneally with a low dose streptozotocin(STZ) dissolved in saline sodium citrate(SSC) repeatly and fed with high-fat food. After establishing DN models in eight weeks, chronobiology disorder were induced by changing illumination rhythm in chronobiology disorder rats and DN chronobiology disorder rats for six weeks. The drinking volume, urine volume and the change of weight were recorded. The blood glucose, 24 h urinary protein excretion, the ratio of cAMP and cGMP were measured, then, to observe the pathological changes of the kidney, histology of the kidney sections stained with hematoxylin-eosin and Periodic Acid-Schiff were used.[Results] ①Compared with those in control group, the drinking volume, urine volume, blood glucose, 24 h urinary protein excretion significantly increased in DN group and DN chronobiology disorder group(P<0.01), the expression of cAMP decreased in DN group(P<0.05, the expression of cGMP increased (P<0.05) and the ratio of cAMP and cGMP significantly decreased (P<0.01) in DN chronobiology disorder group.②In contrast to the mice in DN group, the drinking volume, urine volume and blood glucose significantly decreased and 24h urinary protein excretion significantly increased (P<0.01), the results of renal pathological showed developed renal lesions, the expression of cGMP increased and the ratio of cAMP and cGMP decreased(P<0.05) in DN chronobiology disorder group.[Conclusion] The disruption of chronobiological rhythm may promote diabetic nephropathy through “deficiency of Qi and Yin” pathogenesis. |
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