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| The pre-explore of rDNA ITS sequence of Flos Rosae Rugosae in Shandong province |
| Hits 1815 Download times 1242 Received:October 20, 2014 |
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| DOI
10.11656/j.issn.1673-9043.2015.02.10 |
| Key Words
Flos Rosae Rugosae;rDNA;ITS sequence |
| Author Name | Affiliation | E-mail | | LI Hong-qin | Heze University, Heze 274015, China | | | MA Chang-hao | Heze Institute of Food and Drug Control, Heze 274000, China | | | PENG Yan-li | Shandong University of Traditional Chinese Medicine, Jinan 250355, China | py1567@163.com |
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| Abstract
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| [Objective] To provide scientific evidence for molecular identification by analyzing ITS sequences in different germplasms from Flos Rosae Rugosae. [Methods] The ITS (Internal Transcribed Spacer) regions were cloned by PCR amplification and sequenced bi-directionally. The ITS sequences were aligned by CLUSTALX(2.0) and genetic distances were calculated. [Results] The genetic distances among different germplasms ranged form 0.000 to 0.011. There were a lot of variable sites in both ITS1 and ITS1 sequences from non-coding regions, as well as 5.8S from the conservative region. [Conclusion] The phylogentic analyses based on ITS sequences can provide reliable molecular evidences for identifying different genotypic germplasms of Flos Rosae Rugosae. |
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