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| Effects of Yanghe Pingchuan granules on the expression of IL-17, TNF-α and IFN-γ in rat asthma serum |
| Hits 1773 Download times 1762 Received:January 31, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2014.06 |
| Key Words
Bronchial asthma;Yanghe Pingchuan granules;Dexamethasone;IL-17;TNF-α;IFN-γ |
| Author Name | Affiliation | E-mail | | DING Wei-wei | Anhui University of Traditional Chinese Medicine, Hefei 230031, China | | | YANG Cheng | First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230038, China | | | XU Zeng-mei | Anhui University of Traditional Chinese Medicine, Hefei 230031, China | | | TONG Jia-bing | First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230038, China | | | LU Mei | Anhui University of Traditional Chinese Medicine, Hefei 230031, China | | | TONG Xiang-li | Anhui University of Traditional Chinese Medicine, Hefei 230031, China | | | LI Ze-geng | Anhui University of Traditional Chinese Medicine, Hefei 230031, China | li6609@126.com |
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| Abstract
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| [Objective] To investigate the effects of Yanghe Pingchuan granules on the expression level of IL-17, TNF-α and IFN-γ in rat asthma serum.[Methods] Ninty SD rats were randomly divided into six groups which were normal group (N group), model group (M group), low, middle and high-dose of Yanghe Pingchuan group (YHPC-L, YHPC-M and YHPC-H group) and dexamethasone group (DXM group). For inducing asthma, the rats were sensitized by intraperitoneal injection with ovalbumin(OVA), and then activated by inhalation. Furthermore, dexamethasone was applied to replicate kidney-deficiency rat asthma model, while the N group were treated with saline as a control. HE staining was used to evaluate the pathological changes of lung tissue in each groups, ELISA was used to detect changes of IL-17, TNF-α and IFN-γ in the rat serum. [Results] The expression of IL-17 and TNF-α is significantly higher in M group than in N group, but IFN-γ is obviously lower. Compared with M group, the levels of IL-17 and TNF-α decreased notably (P<0.01), while IFN-γ levels increased (P<0.01). The effect of Yanghe Pingchuan Granules on IL-17 and TNF-α is lower than DXM (P<0.01), whereas is stronger than DXM on IFN-γ(P<0.01).[Conclusion] Yanghe Pingchuan granules could cure bronchial asthma by anti-inflammatory, enhancing immunity and reducing pathological changes. |
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