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| Method of LC-MS for the determination of glycyrrhetinic acid in rat plasma and pharmacokinetic study of glycy rrhetinic acid |
| Hits 1815 Download times 1444 Received:April 15, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2015.04.10 |
| Key Words
Sanao decoction;glycyrrhetinic acid;pharmacokinetic |
| Author Name | Affiliation | E-mail | | WANG Xing-rui | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | OUYANG Hui-zi | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | DOU Ting | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | BO Fang | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | TU Ya-ru | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Jun | Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | hejun673@163.com |
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| Abstract
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| [Objective] To develop a LC-MS method for the pharmacokinetic study of glycyrrhetinic acid after administration of Sanao decoction. [Methods] The method of determination was performed on Agilent Zorbax SB-C18 (4.6 mm×150 mm, 5 μm) column using acetonitrile and water containing of 10 mM Ammonium formate as mobile phase in isocratic elution with 90:10. The flow rate was 0.3 mL/min and the temperature was 25 ℃. Electrospray ionization(ESI) source was applied and operated in the negative ion mode with SIM scanning mode. The quantitative ions of glycyrrhetinic acid and ursolic acid were m/z 469.4 and m/z 455.4. Rats were oral administration with single dose Sanao decoction concentrated solution. The plasma samples were through liquid-liquid extraction pretreatment. Using the method of LC-MS for the determination of plasma concentration of glycyrrhetinic acid and calculate the pharmacokinetic parameters by DAS(ver.1.0).[Results] The linear rang of glycyrrhetinic acid was 5~10 000 ng/mL and it's limit of quantity was 5 ng/mL. Endogenous substances did not interfere with the determination of glycyrrhetinic acid and internal standard. The methods of precision, accuracy, recovery and stability are in line with the requirements of determination of biological samples and may be applied for glycyrrhetinic acid pharmacokinetic study. [Conclusion] The method has the characteristics of high specificity, high accuracy, good sensitivity, and can be used for glycyrrhetinic acid pharmacokinetic study. |
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