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| Protective effect of Xinnao Shutong on hypoxia/reoxygenation injured nerve cells and its mechanism |
| Hits 1570 Download times 2188 Received:March 14, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2015.04.14 |
| Key Words
Xinnao Shutong;neuroprotection;hypoxia/reoxygenation;Neuro-2a |
| Author Name | Affiliation | E-mail | | MA Meng-meng | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HU Li-min | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Qiao-yue | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YANG Hong-yun | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Xu-mei | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | GUO Hong | Institute of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | cacti1983@163.com |
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| Abstract
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| [Objective]To study the protective effect of Xinnao Shutong on hypoxia/reoxygenation injured nerve cells and its mechanism. [Methods] Neuro-2a cells were cultured in vitro, divided into control, model, low-dose, mid-dose and high-dose Xinnao Shutong (10、25、50 g/mL) groups. Establishmenting OGD 4 h/Rep 24 h damage model, detecting cell viability, lactate dehydrogenase (LDH) leakage, reactive oxygen species(ROS)level and mitochondrial membrane potential (△Ψm). [Results] Compared with model group Xinnao Shutong can significantly increase the Neuro-2a cell activity, reduce the leakage of LDH, decrease the level of ROS, enhance the mitochondrial membrane potential. [Conclusion] Xinnao Shutong has obvious protective effect on hypoxia/reoxygenation injured nerve cells, and the mechanism is related to influence ROS level and mitochondrial membrane potential on Neuro-2a. |
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