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| Effect of Xinfukang combined with Bone marrow mesenchymal stem cells on the expression of CD31 and VEGFR2 in cardiac stem cells |
| Hits 1610 Download times 1782 Received:June 03, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2015.05.05 |
| Key Words
Xinfukang;BMSC;CSC;CD31;VEGFR2 |
| Author Name | Affiliation | E-mail | | WANG Yi-jing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZENG Wen-yun | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Hu-hu | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WEI Bing | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | KAN Bo-hong | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | FAN Ying-chang | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | fych@tjutcm.edu.cn |
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| Abstract
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| [Objective] To observe the regulation of CD31 and VEGFR2 protein expression in cardiac stem cells (CSC) under the the synergy between Xinfukang and bone marrow mesenchymal stem cells (BMSC). [Methods] The concentration of SDF-1α from BMSC with Xinfukang were detected by Elisa. The CSC was obtained through tissue explant method, and the purity was evaluated by flow cytometry and immunofluorescence. During the experiment, the CSC pretreated with AMD3100 were co-cultured with Xinfukang,72 h later, the protein expression of CD31 and VEGFR2 in CSC was detected by Western Blot. [Results] Compared with the other two groups, the CD31 and VEGFR2 protein expression of CSC in the Xinfukang group was significantly increased (P<0.05). [Conclusion] BMSC with Xinfukang can increase the protein expression of CD31 and VEGFR2 in CSC,and the mechanism may be related to SDF-1α/CXCR4 axis. |
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