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| The improved experiment of phagocytic function of peritoneal macrophages in mice |
| Hits 2913 Download times 5864 Received:July 25, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2015.05.06 |
| Key Words
macrophages;phagocytosis;saccharomycetes |
| Author Name | Affiliation | E-mail | | ZHOU Hui-fang | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193 | | | XU Qian | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193 | | | BIAN Yu-hong | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193 | | | LIU Xiao-hui | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193 | | | LI Dan | School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193 | danlee10@126.com |
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| Abstract
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| [Objective] The use of saccharomycetes instead of chicken red blood cell in phagocytosis test, glucosinolates glycolic acid sodium as macrophages stimulants, observe the phenomenon and the function of phagocytosis of mouse peritoneal macrophages in vivo, and explore and improve the experiment method.[Methods] Mice were injected of 3% sodium thioglycolate solution by intraperitoneal induced macrophages in the peritoneal, chicken red blood cells suspension and saccharomycetes suspension were injected by intraperitoneal injection after 96 h, and 10, 30 and 60min later, observed the phenomenon of in macrophage phagocytosis of chicken red blood cells and saccharomycetes, and determine the phagocytic percentage and phagocytic index. [Results] The phenomenon of mouse macrophage phagocytosis of chicken red blood cells and saccharomycetes can be observed after 30 min, the mean phagocytic percentage of chicken red blood cells and saccharomycetes were 15.3% and 18.9% respectively, the phagocytic index of the chicken red blood cells and saccharomycetes were 19.3 and 45.9 respectively. The use of saccharomycetes as consuming particles can increase the phagocytic percentage and the phagocytic index.[Conclusion] The phenomenon of mouse peritoneal macrophage phagocytosis of saccharomycetes was more obvious in vivo, and the phagocytosis capacity is larger, but also save the time for preparation of chicken red blood cells, the operation was more convenient. |
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