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| Study on purification of the total flavonoids from leaves of Magnolia officinalis var. biloba |
| Hits 1685 Download times 1716 Received:May 25, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2015.05.08 |
| Key Words
leaves of Magnolia officinalis var. biloba.;total flavonoids;purification;antioxidant activity |
| Author Name | Affiliation | E-mail | | YI Jun | Department of Chemistry and Life Science, Fujian Institute of Education, Fuzhou 350025, China | | | WU Yan-bin | Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China | wxsq1@163.com | | WU Jin-yu | Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China | | | WANG Tao | Research Institute of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | wangt@263.net |
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| Abstract
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| [Objective] To purify total flavonoids from leaves of Magnolia officinalis var. biloba. [Methods] The effect of macroporous resin static and dynamic adsorption on the purity of total flavonoids from leaves of Magnolia officinalis var. biloba. was carried out. Before and after purification of total flavonoids, their purities and antioxidant activities using DPPH and ABTS models in vitro were compared. [Results] The best macroporous resin was HPD722 resin that had good effect on purification of total flavonoids. The best purification was 0.12 g/mL sample concentration and 2 BV/h. The sample volume was 5.5 times the column volume. 10% EtOH of 3.5 times the column volume was used to remove the impurity, and then, 50% EtOH of 4 times the column volume was used to elute. The purity of total flavonoids of 75% EtOH extracts from leaves of Magnolia officinalis var. biloba.was purified from 27.44% to 61.67%. The scavenging ability (IC50) of DPPH free radical and ABTS free radical were from 44.88±1.31 μg/mL to 19.97±0.85 μg/mL and from 592.2±13.14 μg/mL to 169.78±0.99 μg/mL, respectively. [Conclusion] HPD722 macroporous resin can be used as the adsorbent to purify the total flavonoids from leaves of Magnolia officinalis var. biloba. This result provides the basis for the development of resources of the total flavonoids from leaves of Magnolia officinalis var. biloba. |
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