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| Effect of Xueshuantong and Ginsenoside Rd on lipopolysaccharides actived microglia cells |
| Hits 1864 Download times 1573 Received:July 13, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2015.06.07 |
| Key Words
Xueshuantong;nitric oxide;inflammatory mediator;microglia |
| Author Name | Affiliation | E-mail | | YANG Hong-yun | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Xiao-lei | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | CHAI Li-juan | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Shao-xia | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | wangshaoxia1978@hotmail.com | | HU Li-min | Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin Key Laboratory of Chinese medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To study the effects of Xueshuantong and its monomer on actived microglia (BV-2) and its mechanism. [Methods] The experiment was divided into control group, lipopolysaccharide(LPS) group, dosing group and positive control group (mino), detecting of Xueshuantong (different dilution multiples of Xueshuantong and its monomer composition) to the vitality of microglia and nitric oxide(NO) induced by LPS using the Greiss method. [Results] Between 0.5~50 μg/mL, Xueshuantong could not inhibit the microglia release NO induced by LPS, among the 5 kinds of monomer composition of Xueshuantong, ginsenoside Rg1, Rb1, Re and Notoginsenoside could not inhibit the microglia release NO induced by LPS, however, ginsenoside Rd could inhibit NO secretion without affecting the cell vitality, and inhibit the mRNA expression of iNOS, IL-1β, IL-6. [Conclusion] The monomer composition of ginsenoside Rd in Xueshuangtong can inhibit activated microglia release NO and the mRNA of iNOS,IL-1β,IL-6 significantly. It may be one of neural protection mechanism. |
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