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| Effect of simvastatin on thrombosis in unstable plaque thrombosis rabbits model |
| Hits 1957 Download times 2310 Received:October 30, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2016.01.06 |
| Key Words
simvastatin;atherosclerosis;plaque instability;thrombosis |
| Author Name | Affiliation | E-mail | | YANG Lin | School of Combination of TCM and WM, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LU Bin | School of Combination of TCM and WM, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | DU Yun | Medical Department, Tianjin Medical College, Tianjin 300222, China | | | GUO Mao-juan | School of Combination of TCM and WM, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Yun-sha | School of Combination of TCM and WM, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LI Hu-hu | School of Combination of TCM and WM, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZENG Wen-yun | School of Combination of TCM and WM, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | MA Dong-ming | School of Combination of TCM and WM, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | JIANG Xi-juan | School of Combination of TCM and WM, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | xijuanjiang@foxmailcom |
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| Abstract
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| [Objective] To investigate the effect of simvastatin on atherosclerotic plaque instability model of thrombosis in rabbits. [Methods] Male New Zealand rabbits were randomly divided into 2 groups. One group (sham group) was always given a normal diet. Another group was fed high-fat diet for four weeks, and then abdominal aortic endothelium was damaged by using 4F Forgarty catheter. The rabbits were replaced for normal diet until being sacrificed, and were randomly divided into model group and simvastatin group. Simvastatin group received simvastatin [10 mg/(kg·d)] for 4 weeks. Sham group and model group were given an equal volume of normal saline. Rabbits were sacrificed after 4 weeks of drug intervention in each group. Rabbits plaque rupture were triggered by giving intraperitoneal injections of venom under (0.15 mg/kg) and the ear vein injection of histamine (0.02 mg/kg) in 48 h and 24 h twice before sacrifice. Rabbits abdominal aortic thrombosis were observed in the stereo microscope after sacrifice. Rabbits were detected vWF and D-Dimer by ELISA, and were detected concentration of TXB2 and 6-keto-PGF1α by radioimmunoassay, and were calculated the ratio of TXB2/6-keto-PGF1α。 [Results] After four weeks of treatment with simvastatin, simvastatin group were observed to reduce plaque site thrombosis under stereo microscope, and there was a significant difference compared with the model group(P<0.01). Plasma levels of vWF: Ag and D-Dimer were lower in the simvastatin group, and there was a significant difference compared with the model group (P<0.01). Plasma concentration of TXB2 was lower and plasma concentration of 6-keto-PGF1α was higher, and there was a significant difference compared with the model group(P<0.05).The ratio of TXB2/6-keto-PGF1α was lower, and there was a significant difference compared with the model group(P<0.01). [Conclusion] Simvastatin can repair damaged endothelial cells and improve blood hypercoagulable state, inhibition of platelet aggregation, and thus play a role in anti-thrombosis. This study provides experimental evidence that simvastatin prevents thrombosis in the clinical for patients with AS unstabled plaque. |
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