|
| The influence of clearing heat and dispersing phlegm method on the pathological lung tissue of AECOPD rat model (syndrome of phlegm and heat) |
| Hits 1719 Download times 1948 Received:September 12, 2015 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2016.01.09 |
| Key Words
clearing heat and dispersing phlegm way;rat model of exacerbation chronic obstructive pulmonary disease (syndrome of phlegm and heat);pathological lung tissue |
| Author Name | Affiliation | | LI Jie | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | WANG Lin-yang | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | CHENG Juan | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | WU Jun | School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China | | QIU Ze-ji | School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China | | LUO Hui | Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
|
| Abstract
|
| [Objective] Based on the previous research foundation that the way of clearing heat and dispersing phlegm can increase the concentration of antibiotic in AECOPD lung tissue, this study will explore the mechanism of action that Louqinzhisoujian improves transport of antibiotics in the lung tissue by the means of intervening AECOPD damaged lung tissue morphology. [Methods] Fivty Wistar male rats were randomly divided into normal group, model control group, ambroxol group, Erchentang group, Louqinzhishoujian group,10 in each group . To establish rat model of AECOPD(syndrome of phlegm and heat) by passive cigarette smoking,intratracheal instillation of lipopolysacchricle(LPY), intranose instillation of staphylococcus aureus, plus put in the Wind-heat oven. Pathological tissue using HE staining, apply image analysis software(IPP6.0) measuring the average lung lining interval(MLI), mean alveolar number(MAN), alveolar area and the total area ratio(PAA); Weigert stained specimen while measuring relative area of elastic fibers. [Results] Pathology results showed : lung tissues structure of model control group were more severe damage than those of normal group, including increase of inflammatory cells. In this way the model successfully reproduced. The lung tissue destructions of Louqinzhishoujian group and ambrohexel group were relatively light, the second group followed Erchentang group. MAN and PAA: Compared with model control group, Louqinzhishoujian group, Erchentang group, and ambroxol group,they were statistically significant(P<0.05). Weigert staining showed the alveolar wall elastic fiber, Louqinzhisou group was the highest of elastic fiber content and model control group was the most serious damage among them, the orders from high to low were Louqinzhisou group, ambroxol group, normal group, Erchentang group and model control group.[Conclusion] The dispersing phlegm ways of TCM and WM also have better protection for damaged lung tissue of AECOPD rat model, the way of clearing heat and dispersing phlegm way is special evident. The mechanism of action that Louqinzhisoujian improves transport of antibiotics in the lung tissue may to some extent improve the damaged structure of pulmonary tissue to reduce the penetration of the antibiotic which helps it to play a role. |
|
|
|
|
|
