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| HSCCC separation and purification gentian alkaline ethanol extracts of gentian |
| Hits 2171 Download times 1974 Received:September 26, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2016.01.12 |
| Key Words
Gentiana scabra Bunge;gentianine;HSCCC |
| Author Name | Affiliation | E-mail | | SHI Yao | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | WANG Lin-lin | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | YU He-shui | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LAI Qing-hai | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | DING Hui | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | ZHANG Li-juan | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | SONG Xin-bo | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | songxinbo@tjutcmeducn |
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| Abstract
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| [Objective] To establish the HSCCC method for separating the gentianine from the alkaline ethanol extracts of gentiana. [Methods] Separation and purification of gentianine by TBE300B HSCCC technology, through the optimization of the solvent system, with n-hexane : ethyl acetate : methanol : water (5:5:4:6) as the solvent system, the upper as stationary phase and the next phase as mobile phase, the rotational speed of the instrument was 850 r/min, the flow rate was 1.5 mL/min, the temperature was 25 ℃, the detection wavelength was 280 nm. [Results] The gentianine was obtained from the gentian alkaline ethanol extracts by D151 cation exchange resin combining with HSCCC, the content was above 98.8%. [Conclusion] The method is stable and has high results, it could be used to separate gentianine efficiently from alkaline ethanol extract of gentiana. |
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