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| Anti-tumor activities research of chiral ruthenium to targeted telomeres G-quadruplex |
| Hits 1591 Download times 1784 Received:November 21, 2015 |
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| DOI
10.11656/j.issn.1673-9043.2016.02.10 |
| Key Words
chiral ruthenium complexes Λ- [Ru (phen) 2p-MOPIP]2+(Λ-OMe);Human gastric cancer cell lineMGC-803;anti-tumor activity |
| Author Name | Affiliation | E-mail | | HUANG Zhu-Juan | Guangzhou University of Traditional Chinese Medcine, Guangzhou 510006 | | | SU Ning | Guangzhou University of Traditional Chinese Medcine, Guangzhou 510006 | | | WU Shao-feng | Guangzhou University of Traditional Chinese Medcine, Guangzhou 510006 | | | LIN Rui-shan | Guangzhou University of Traditional Chinese Medcine, Guangzhou 510006 | | | GUO Jian-chao | Guangzhou University of Traditional Chinese Medcine, Guangzhou 510006 | | | ZHANG Xiao-nian | Guangzhou University of Traditional Chinese Medcine, Guangzhou 510006 | shezxn84@gzucm.edu.cn |
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| Abstract
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| [Objective] Study on anti-tumor activities research of a paire of chiral ruthenium complexes Λ-[Ru(bpy)2(pyip)]2+(Λ-OMe)、Δ-[Ru(bpy)2(pyip)]2+(Δ-OMe)and their racemic mixture [Ru(phen)2p-MOPIP]2+(dl-OMe) in vitro experiments. [Methods] Three complexes were acting 48 hours on human gastric cancer cell line (MGC-803), human colon cancer cell lines (Colo205), human breast cancer cell line (MCF-7), human lung adenocarcinoma epithelial cell line (A549), human hepatocellular carcinoma cell line (HepG2), human tongue squamous cell carcinoma lines (SCC-9), human gastric epithelial cell line (GES-1) and cisplatin (CDDP) is a positive control.And then find the drug and tumor cells which half inhibitory concentration (IC50) was the smallest by MTT assay. Second, Detect the drug's inhibitory effect on tumor cells after drug treatment 24 、48、72 hours. Third, induced apoptosis of gastric cancer cells with Hoechest33342 staining. [Results] The best anti-tumor effects drug and cell is chiral ruthenium complexes Λ-[Ru (phen)2p MOPIP]2+(Λ-OMe) and MGC-803 with MTT assay. The IC50 is:7.4 μg/mL at 48 hours.The tested compound induces cell death in a dose and time dependent manner on MGC-803 cells; Hoechest33342 staining showed that with the increase of drug concentration,more obvious of apoptosis after 48 hours drug treatment. [Conclusion] Chiral ruthenium complexes have good anti-tumor activity and the Λ-OMe have significant anti-tumor activities. |
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