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The orthogonal experiment method to optimize the extraction process of senna
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DOI   10.11656/j.issn.1673-9043.2016.02.13
Key Words   senna;sennoside;sennoside A;sennoside B;extraction process
Author NameAffiliationE-mail
JIN Shi-shi Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China  
NIU Huan-yun Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China  
DING Hui Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China  
WU Jie Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China  
YU He-shui Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China  
LI Wei Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China  
CAO Man Tianjin Zhongyi Pharmaceutical Co.Ltd,Tianjin 300193, China  
SONG Xin-bo Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Tianjin Zhongyi Pharmaceutical Co.Ltd,Tianjin 300193, China 
songxinbo@tjutcm.edu.cn 
Abstract
    [Objective] To select the best extraction process of sennoside from senna. [Methods] To establish the method for simultaneous determination of Sennoside A and Sennoside B, the method utilizes the content of Sennoside A and Sennoside B as a quality control. Based on the single factor, the best extraction process is selected by orthogonal test. [Results] The best process for extraction was 10 fold 0.5% sodium bicarbonate was used to extract Sennoside under 60 ℃ for an hour. [Conclusion] The developed process is stable and reliable, and can be readily utilized for related research and actual production.

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