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| Effect of Astragals and Angelica Mixture on renal expression of NF-κB and MCP-1 in rats with diabetic |
| Hits 1604 Download times 1754 Received:February 21, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2016.03.06 |
| Key Words
astragals and angelica mixture;diabetic nephropathy;inflammation;NF-κB;MCP-1 |
| Author Name | Affiliation | E-mail | | WANG Xiu-ping | The Second Clinical College of Wuhan University, Wuhan 430030, China | | | REN Xiao-dan | The Second Clinical College of Wuhan University, Wuhan 430030, China | | | ZHANG Ying-wen | The Second Clinical College of Wuhan University, Wuhan 430030, China | hhao3838@sina.com |
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| Abstract
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| [Objective] To investigate the anti-inflammation mechanism of Astragals and Angelica Mixture with an influence on the expression of inflammatory factors NF-κB and MCP-1, and its correlation with renal protective effect. [Methods] Using STZ+high-fat diet to establish animal models of DN. Enzyme coupling colorimetric method to detect the serum glucose, lipids and the renal function related parameters. Kidney structure change was observed under the light and electron microscope, Enzyme-linked immune sorbent assay to detect the protein content of NF-κB and MCP-1 in the kidney. Real-time fluorescent quantitative PCR to measure the change of NF-κB and MCP-1 mRNA expression. [Results] The protein and mRNA expression of NF-κB and MCP-1 in DN rats kidney was significantly increased. After treated with Astragalus and Angelica Mixture, the renal function was improved, kidney protein and mRNA expression of NF-κB, MCP-1 was significantly reduced. [Conclusion] Astragals and Angelica Mixture can effectively decrease the protein and mRNA expression and activity of NF-κB and MCP-1 in DN rats kidney, ameliorate kidney damage, have a anti-inflammation effect. |
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