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| Protective effect of Heying Anxin formula on anti-inflammatory and promoting angiogenesis |
| Hits 1345 Download times 1248 Received:February 17, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2016.03.08 |
| Key Words
Heying Anxin formula;vascular smooth muscle cells;aging rats;cardiovascular system |
| Author Name | Affiliation | E-mail | | HE Wei | Hebei North University, Zhangjiakou 075029, China | | | LI Yong-min | Hebei North University, Zhangjiakou 075029, China | liyongmin2001@sina.com | | ZHANG Hai-xia | Hebei North University, Zhangjiakou 075029, China | | | CHEN Xi-long | Hebei North University, Zhangjiakou 075029, China | | | SU Zhi-yuan | Hebei North University, Zhangjiakou 075029, China | |
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| Abstract
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| [Objective] To investigate protective effects and mechanisms of Heying Anxin formula on the cardiovascular system of aging rats. [Methods] Rat vascular smooth muscle cells were incubated by H2O2(200 μmol/L) for 2 h as inflammation model group, same dose of H2O2 was administered 2 h after treatment with Heying An xin formula(100 μg/mL, 50 μg/mL, 25 μg/mL)as therapeutic group and incubated for another 24 h, content of NO were measured. Subcutaneous injection in neck and back of D-galactose(125 mg/kg) to establish aging rats model. Model group: D-galactose(125 mg/kg), Heying Anxin formula (500 mg/kg+D-galactose 125 mg/kg, 250 mg/kg+D-galactose 125 mg/kg、125mg/kg+D-galactose 125 mg/kg) as treating groups, as well as normal group and young group. The RT-PCR technique was used to measure the expression of relevant genes, such as nitrous oxide system(NOS) and basic fibroblast growth factor (b-FGF) in the tissues of brain and liver. [Results] Heying Anxin formula can significantly decrease the content of NO(P<0.05 vs control group), also compared with that of control group, He ying Anxin formula can significantly decrease NOS expression and enhance b-FGF expression in rats brain and liver tissues (P<0.01). [Conclusion] Heying Anxin formula has greatly protective effects on anti-inflammatory and cardiovascular regeneration of aging rats, and it may be take effect by the brain and liver. |
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